RNAseq from olive xylem: Sample Olive Xylem OG3 - SRA

SRX1669652: RNAseq from olive xylem: Sample Olive Xylem OG3
1 ILLUMINA (Illumina HiScanSQ) run: 22.4M spots, 1.1G bases, 741Mb downloads

Design: Total RNAs were extracted from 1 g of xylem tissues powdered in liquid nitrogen. Libraries of xylem-derived mRNAs were synthesized from three infected cvs Leccino and Ogliarola salentina plants and two healthy plants of the same cultivars. A total amount of 3µg mRNA per sample was used for preparation of libraries that were generated using the TruSeq RNA Sample Preparation Kit v2 (Illumina, USA) following manufacturer’s recommendations. Quality of the synthesized libraries was assessed by the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). Cluster generation and 50 bp single-end sequencing were performed on a cBot apparatus and HiScanSQTM platform (Illumina, USA).

Submitted by: CNR-Institute for Sustainable Plant Protection

Study: Olea europaea cultivar:Leccino, Ogliarola salentina Transcriptome or Gene expression

PRJNA316374 • SRP072340 • All experiments • All runs

show Abstracthide Abstract

Transcriptome profiling of two olive cultivars showing differential phenotypes in response to infection by the CoDiRO strain of Xylella fastidiosa subsp. pauca to investigate the molecular mechanisms underlying the different olive plant responses to the pathogen.

Sample:

SAMN04579667 • SRS1361213 • All experiments • All runs

Organism: Olea europaea

Library:

Name: Olive Xylem OG3

Instrument: Illumina HiScanSQ

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 22.4M spots, 1.1G bases, 741Mb

Run	# of Spots	# of Bases	Size	Published
SRR3310960	22,406,467	1.1G	741Mb	2016-06-29

ID:: 2395420

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