Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain

Text - scientific article/review article


Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for R-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.


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  • Xylella fastidiosa


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