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A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apul...
Development of an FTP-LAMP assay based on TaqMan real-time PCR and LAMP for the specific detection of Xylella fastidiosa De Donno and mulberry strains in both plants and insect vectors
We developed two real-time detection assays, TaqMan real-time PCR and LAMP, using primers and probe designed based on a sequence annotated to code for a Haemagglutinin-related protein (Hg) of Xyl...
Direct tissue blot immunoassay for detection of Xylella fastidiosa in olive trees
A direct tissue blot immunoassay (DTBIA) technique has been compared with ELISA and PCR for detection of Xylella fastidiosa in olive trees from Apulia (southern Italy). Fresh cross-sections of youn...
Identification of three potential insect vectors of Xylella fastidiosa in southern Italy
In order to identify potential vectors of Xylella fastidiosa in olive orchards in Puglia (southern Italy), Hemiptera insects were collected from October to December, 2013, in olive orchards with hi...
Multilocus sequence typing of Xylella fastidiosa isolated from olive affected by "olive quick decline syndrome" in Italy
The recent finding of Xylella fastidiosa (Xf) in olive trees in southern Italy, the scanty molecular information on this bacterium and its association with the olive quick decline syndrome (OQDS) p...
On-site detection of Xylella fastidiosa in host plants and in "spy insects" using the real-time loop-mediated isothermal amplification method
A recent severe outbreak of Xylella fastidiosa associated with 'olive quick decline syndrome' (OQDS) was reported in Apulia (Southern Italy). In this study an on-site real-time loop-mediated isothe...
