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16S-23S RDNA: Polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S- 23S rDN...
Accumulation of Azelaic Acid in Xylella fastidiosa-Infected Olive Trees: A Mobile Metabolite for Health Screening
Monitoring Xylella fastidiosa is critical for eradicating or at least containing this harmful pathogen. New low-cost and rapid methods for early detection capability are very much needed. Metabolom...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...
A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apul...
A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa
A simplified protocol of subtractive hybridization based on the technique of L. M, Kunkel, A. P, Monaco, W, Middlesworth, H, D, Ochs & S, A. Latt (1985, Proc Natl Acad Sci USA, 82, 4778-4782) was u...
Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae
The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis...
A survey of potential insect vectors of the plant pathogenic bacterium Xylella fastidiosa in three regions of Spain
The emergence of a rapid-spreading olive disease associated with Xylella fastidiosa in southern Italy represents a high risk to susceptible crops in other countries of the Mediterranean basin, if i...
Budding method affects transmission of Xylella fastidiosa in plum
Detection of Xylella fastidiosa Wells et al. by enzyme-linked immunosorbent assay indicated that plums (Prunus hybrids) had higher absorbance values than peaches [Prunus persica (L.) Batsch]. The s...
Categorisation of plants for planting, excluding seeds, according to the risk of introduction of Xylella lfastidiosa
Following a request from the European Commission, EFSA was asked to provide urgent technical assistance in the field of plant health as regards the list of host plants of Xylella fastidiosa reporte...
Combined use of a new SNP-based assay and multilocus SSR markers to assess genetic diversity of Xylella fastidiosa subsp pauca infecting citrus and coffee plants
Two haplotypes of Xylella fastidiosa subsp. pauca (Xfp) that correlated with their host of origin were identified in a collection of 90 isolates infecting citrus and coffee plants in Brazil, based ...
Comparative analysis for quantification of Citrus Variegated Chlorosis in the field
Citrus Variegated Chlorosis (CVC) caused by Xylella fastidiosa is one of the most important diseases for Brazilian citriculture. The CVC is a vascular disease with a long incubation period and symp...
