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A delivery system for field application of paratransgenic control
As an alternative to chemical pesticides, paratransgenesis relies on transformation of symbiotic bacteria of an arthropod vector to deliver molecules that disrupt pathogen transmission. For over a ...
An engineered innate immune defense protects grapevines from Pierce disease
We postulated that a synergistic combination of two innate immune functions, pathogen surface recognition and lysis, in a protein chimera would lead to a robust class of engineered antimicrobial th...
Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.
The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM) bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one ca...
BREEDING, GENETIC AND GENOMIC OF CITRUS FOR DISEASE RESISTANCE
Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture rega...
Chromosome-Based Genetic Complementation System for Xylella fastidiosa
Xylella fastidiosa is a xylem-limited, gram-negative bacterium that causes Pierce's disease of grapevine. Here, we describe the construction of four vectors that facilitate the insertion of genes i...
Effects of codon bias on heterologous expression of fluorescent proteins in Xylella fastidiosa
The xylem pathogenic bacterium Xylella fastidiosa is characterized by slow growth, a small genome, and low codon usage bias in protein-coding genes. In most organisms, genes have bias in codon usag...
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformatio...
High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X-campestris pv. campestris generated by an efficient transposon mutagenesis system
A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highl...
Insights into the Activity and Substrate Binding of Xylella fastidiosa Polygalacturonase by Modification of a Unique QMK Amino Acid Motif Using Protein Chimeras
Polygalacturonases (EC 3.2.1.15) catalyze the random hydrolysis of 1, 4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. Xylella fastidiosa possesses a single polygalacturonase...
Stable transformation of the Xylella fastidiosa citrus variegated chlorosis strain with oriC plasmids
Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e,g,, grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fasti...
Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047
Xylella fastidiosa (Xf) is a fastidious, xylem-inhabiting, Gram-negative bacterium that causes serious plant diseases in a wide range of plant species. The most serious diseases are Pierce's Diseas...
Transgenic banana plants expressing Xanthomonas wilt resistance genes revealed a stable non-target bacterial colonization structure
Africa is among the continents where the battle over genetically modified crops is currently being played out. The impact of GM in Africa could potentially be very positive. In Uganda, researchers ...