As a first approach to generate mutations by DNA insertion, we have developed a shuttle vector, called pSP3. which replicates both in Escherichia coli and in Xylella. Vector pSP3 was constructed by...

Xylella fastidiosa, a pathogen of citrus, is the first plant pathogenic bacterium for which the complete genome sequence has been published. Inspection of the sequence reveals high relatedness to m...