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A new regulatory DNA motif of the gamma subclass proteobacteria: identification of the LexA protein binding site of the plant pathogen Xylella fastidiiosa
Escherichia coli LexA protein is the repressor of a gene network whose members are directly involved in the repair of damaged DNA and in the survival of bacterial cells until DNA lesions have been ...
Citrus genomics
Citrus fruits (sweet orange, mandarin, pummelo, grapefruit, lemon, lime and assorted hybrids) are among the most widely grown and economically important fruit tree crops in the world. As fresh frui...
Directed gap closure in large-scale sequencing projects
A problem in many sequencing projects is the Final closure of gaps left in the clone libraries, which serve as templates for sequencing, because of uncloned or unclonable genomic areas. By use of t...
Evaluation of Xylella fastidiosa genetic diversity by fAFLP markers
The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures ...
HecA, a member of a class of adhesins produced by diverse pathogenic bacteria, contributes to the attachment,, aggregation, epidermal cell killing, and virulence phenotypes of Erwinia chrysanthemi EC16 on Nicotiana clevelandii seedlings
Erwinia chrysanthemi is representative of a broad class of bacterial pathogens that are capable of inducing necrosis in plants. The E. chrysanthemi EC16 hecA gene predicts a 3,850-aa member of the ...
High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X-campestris pv. campestris generated by an efficient transposon mutagenesis system
A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highl...
Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri
The recently sequenced genome of the bacterial plant pathogen Xanthomonas axonopodis pv. citri contains two virB gene clusters, one on the chromosome and one on a 64-kb plasmid, each of which codes...
Lessons from One Fastidious Bacterium to Another: What Can We Learn about Liberibacter Species from Xylella fastidiosa
Huanglongbing is causing economic devastation to the citrus industry in Florida, and threatens the industry everywhere the bacterial pathogens in the Candidatus Liberibacter genus and their insect ...
Microarray analyses of Xylella fastidiosa provide evidence of coordinated transcription control of laterally transferred elements
Genetically distinct strains of the plant bacterium Xylella fastidiosa (Xf) are responsible for a variety of plant diseases, accounting for severe economic damage throughout the world. Using as a r...
Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control
Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. In our previous reports, we revealed the molecular mechanisms of host plant responses that ...
New protein-protein interactions identified for the regulatory and structural components and substrates of the type III secretion system of the phytopathogen Xanthomonas axonopodis pathovar citri
We have initiated a project to identify protein-protein interactions involved in the pathogenicity of the bacterial plant pathogen Xanthomonas axonopodis pv. citri. Using a yeast two-hybrid system ...
Phylogeny of Replication Initiator Protein TrfA Reveals a Highly Divergent Clade of Incompatibility Group P1 Plasmids
Incompatibility group P1 (IncP-1) plasmid diversity was evaluated based on replication initiator protein (TrfA) phylogeny. A new and highly divergent clade was identified. Replication assays indica...