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A Short Protocol for Gene Knockout and Complementation in Xylella fastidiosa Shows that One of the Type IV Pilin Paralogs (PD1926) Is Needed for Twitching while Another (PD1924) Affects Pilus Number and Location

Twitching motility is one of the major virulence factors of the plantpathogenic bacterium Xylella fastidiosa, and it is mediated by type IV pili (TFP) that are present at one of the cell poles. Gen...

Autoaggregation of Xylella fastidiosa cells is influenced by type I and type IV pili

Autoaggregation of widely dispersed Xylella fastidiosa cells into compact cell masses occurred over a period of hours following 7 to 11 days of growth in microfluidic chambers. Studies involving th...

BACTERIAL DISEASES THAT MAY OR DO EMERGE, WITH (POSSIBLE) ECONOMIC DAMAGE FOR EUROPE AND THE MEDITERRANEAN BASIN: NOTES ON EPIDEMIOLOGY, RISKS, PREVENTION AND MANAGEMENT ON FIRST OCCURRENCE

Bacterial diseases are difficult to control (both chemically and biologically), and are restrained primarily by preventive measures. Most important risk factors for the introduction or spread of ba...

Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Flavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. The natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture sys...

Budding method affects transmission of Xylella fastidiosa in plum

Detection of Xylella fastidiosa Wells et al. by enzyme-linked immunosorbent assay indicated that plums (Prunus hybrids) had higher absorbance values than peaches [Prunus persica (L.) Batsch]. The s...

Characterization of the Xylella fastidiosa PD1671 Gene Encoding Degenerate c-di-GMP GGDEF/EAL Domains, and Its Role in the Development of Pierce's Disease

Xylella fastidiosa is an important phytopathogenic bacterium that causes many serious plant diseases including Pierce's disease of grapevines. X. fastidiosa is thought to induce disease by colonizi...

Differentiation of Xylella fastidiosa Strains via Multilocus Sequence Analysis of Environmentally Mediated Genes (MLSA-E)

Isolates of the plant pathogen Xylella fastidiosa are genetically very similar, but studies on their biological traits have indicated differences in virulence and infection symptomatology. Taxonomi...

Disruption of Xylella fastidiosa CVC gumB and gumF genes affects biofilm formation without a detectable influence on exopolysaccharide production

Xylella fastidiosa causes citrus variegated chlorosis (CVC), a destructive disease of citrus. Xylella fastidiosa forms a biofilm inside plants and insect vectors. Biofilms are complex structures in...

Expression of Xylella fastidiosa Fimbrial and Afimbrial Proteins during Biofilm Formation

Complete sequencing of the Xylella fastidiosa genome revealed characteristics that have not been described previously for a phytopathogen. One characteristic of this genome was the abundance of gen...

Factors Affecting the Initial Adhesion and Retention of the Plant Pathogen Xylella fastidiosa in the Foregut of an Insect Vector

Vector transmission of bacterial plant pathogens involves three steps: pathogen acquisition from an infected host, retention within the vector, and inoculation of cells into susceptible tissue of a...

Fast Detection of Olive Trees Affected by Xylella Fastidiosa from UAVs Using Multispectral Imaging

Xylella fastidiosa(Xf) is a well-known bacterial plant pathogen mainly transmitted by vector insects and is associated with serious diseases affecting a wide variety of plants, both wild and cult...

Grape Cultivar and Sap Culture Conditions Affect the Development of Xylella fastidiosa Phenotypes Associated with Pierce's Disease

Xylella fastidiosa is a xylem-limited bacterium in plant hosts and causes Pierce's disease (PD) of grapevines, which differ in susceptibility according to the Vitis species (spp.). In this work we ...