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Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae

The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis...

DETECTION AND IDENTIFICATION OF PHYTOPATHOGENIC XANTHOMONAS STRAINS BY AMPLIFICATION OF DNA-SEQUENCES RELATED TO THE HRP GENES OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA

Three pairs of oligonucleotide primers specific for different regions of the hrp gene (hypersensitive reaction and pathogenicity) cluster of Xanthomonas campestris pv. vesicatoria were designed and...

DEVELOPMENT OF A FAST AMPLIFYRP ACCELER8 DIAGNOSTIC ASSAY FOR GRAPEVINE RED BLOTCH VIRUS

Polymerase chain reaction (PCR) is routinely used for the detection of grapevine red blotch virus (GRBV), a virus with a circular single-stranded DNA genome, in grapevine tissue. Since the preparat...

Development of a real-time PCR-based method for detection of Xylophilus ampelinus

A real-time PCR MGB-probe-based detection method specific to Xylophilus ampelinus, the cause of grapevine bacterial blight, was developed. Used in combination with the DNeasy plant mini kit, the se...

Genetic relationships among strains of Xanthomonas fragariae based on random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacterial repetitive intergenic consensus PCR data and generation of multiplexed PCR primers useful for the identification of this phytopathogen

Genetic relationships among 25 isolates of Xanthomonas fragariae from diverse geographic regions were determined by three PCR methods that rely on different amplification priming strategies: random...

GRAPEVINE NECROTIC UNION, A NEWLY RECOGNIZED DISEASE OF UNKNOWN ETIOLOGY IN GRAPEVINES GRAFTED ON 110 RICHTER ROOTSTOCK IN CALIFORNIA

In Northern California, surveys of several vineyards planted to Vitis vinifera cv. Pinot noir (PN) clones 02A, 667, 777, and UCD 04 grafted onto the rootstock V berlandieri x V rupestris 110 Richte...

Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture

It has been postulated that bacteria attached to the surface of prosthetic hip joints can cause localised inflammation, resulting in failure of the replacement joint. However, diagnosis of infectio...

One Bacterial Cell, One Complete Genome

While the bulk of the finished microbial genomes sequenced to date are derived from cultured bacterial and archaeal representatives, the vast majority of microorganisms elude current culturing atte...

On-site detection of Xylella fastidiosa in host plants and in "spy insects" using the real-time loop-mediated isothermal amplification method

A recent severe outbreak of Xylella fastidiosa associated with 'olive quick decline syndrome' (OQDS) was reported in Apulia (Southern Italy). In this study an on-site real-time loop-mediated isothe...

Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts

Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa...