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A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...
Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae
The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis...
Combination of Chromogenic Differential Medium and estA-Specific PCR for Isolation and Detection of Phytopathogenic Xanthomonas spp.
A xanthomonad differential medium (designated Xan-D medium) was developed, on which streaks and colonies of xanthomonads, including 13 species of the genus Xanthomonas, turned wet-shining yellow-gr...
Detection and analysis of the bacterium, Xylella fastidiosa, in glassy-winged sharpshooter, Homalodisca vitripennis, populations in Texas
The glassy-winged sharpshooter, Homalodisca vitripeninis Germar (Hemiptera: Cicadellidae), is a xylophagous insect that is an endemic pest of several economically important plants in Texas. H. vitr...
DETECTION AND IDENTIFICATION OF PHYTOPATHOGENIC XANTHOMONAS STRAINS BY AMPLIFICATION OF DNA-SEQUENCES RELATED TO THE HRP GENES OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA
Three pairs of oligonucleotide primers specific for different regions of the hrp gene (hypersensitive reaction and pathogenicity) cluster of Xanthomonas campestris pv. vesicatoria were designed and...
Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence
Immunofluorescence detection was performed by tissue sectioning and membrane entrapment of Xylella fastidiosa from the inoculated hybrid selection F8909-08 (Vitis rupestris A. de Serres x V. arizon...
DETECTION OF XYLELLA FASTIDIOSA IN OLIVE TREES BY MOLECULAR AND SEROLOGICAL METHODS
Xylella fastidiosa has recently been identified in the Apulian province of Lecce (south-eastern Italy) in olive trees affected by a devastating disease denoted Olive Quick Decline Syndrome (OQDS), ...
Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction
A sensitive and specific assay for detecting Xylella fastidiosa in potential insect vectors was developed. This assay involves immunomagnetic separation of the bacteria from the insect, followed by...
Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp pauca strain CoDiRO
Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its t...
DEVELOPMENT OF A POLYMERASE CHAIN-REACTION PROTOCOL FOR DETECTION OF XYLELLA-FASTIDIOSA IN PLANT-TISSUE
A 7.4-kb EcoRI fragment of genomic DNA of Xylella fastidiosa strain PCE-RR (ATCC 35879) was used as a probe and was conserved in 18 strains of Xylella. The nucleotide sequence of a 1.0-kb internal ...
Development of a real-time PCR-based method for detection of Xylophilus ampelinus
A real-time PCR MGB-probe-based detection method specific to Xylophilus ampelinus, the cause of grapevine bacterial blight, was developed. Used in combination with the DNeasy plant mini kit, the se...
Fast, easy and efficient DNA extraction and one-step polymerase chain reaction for the detection of Xylella fastidiosa in potential insect vectors
A quick, simple and efficient procedure for detecting Xylella fastidiosa in potential insect vectors is described. The procedure employs a commercially available DNeasy tissue kit for the extractio...
