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Advances and innovative concepts to control Xylella fastidiosa colonization in citrus plants

Xylella fastidiosa is responsible for diseases that affect different crops worldwide. One of them is Citrus Variegated Chlorosis (CVC), which results from the xylem vessels occlusion by X. fastidio...

Analysis of Defense-Related Gene Expression in Citrus Hybrids Infected by Xylella fastidiosa

Resistance to Xylella fastidiosa was evaluated in 264 hybrids of crosses between Murcott tangor (Citrus reticulata x Citrus sinensis) and Pera sweet orange (C. sinensis) under field conditions. Uni...

Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa

In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two ...

Analysis of resistance to Xylella fastidiosa within a hybrid population of Pera sweet orange x Murcott tangor

Resistance to Xylella fastidiosa was evaluated within a population of 20 interspecific hybrids of Pera sweet orange and Murcott tangor under greenhouse conditions. Efficiency of inoculation, multip...

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers

Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...

A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

A simplified protocol of subtractive hybridization based on the technique of L. M, Kunkel, A. P, Monaco, W, Middlesworth, H, D, Ochs & S, A. Latt (1985, Proc Natl Acad Sci USA, 82, 4778-4782) was u...

Bacterial diversity in potential cicadellids vectors of Xylella fastidiosa Wells et al. in coffee plants in Puerto Rico

Bacteria from head and body tissues of three potential insect vectors of Xylella fastidiosa (Xf), Apogonalia spp., Hortensia similis and Caribovia coffeacola, were isolated in periwinkle wilt (PW) ...

Bioassay assessment of Metarhizium anisopliae (Metchnikoff) Sorokin (Deuteromycota : Hyphomycetes) against Oncometopia facialis (Signoret) (Hemiptera : Cicadellidae)

Citrus Variegated Chlorosis ( CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the effica...

Biocontrol of Pierce's disease of grapevine and citrus greening with a benign strain of Xylella fastidiosa

Vascular diseases caused by xylem-limited Xylella fastidiosa subspp. and phloem-limited Candidatus Liberibacter spp. result in large economic losses in many agricultural plants. X. fastidiosa is ra...

Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.

The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM) bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one ca...

BREEDING, GENETIC AND GENOMIC OF CITRUS FOR DISEASE RESISTANCE

Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture rega...

Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene

The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceralde...