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ACQUISITION AND RETENTION OF XYLELLA-FASTIDIOSA BY AN EFFICIENT VECTOR, GRAPHOCEPHALA-ATROPUNCTATA
The blue-green sharpshooter (BGSS), Graphocephala atropunctata (Signoret), is an efficient vector of Xylella fastidiosa Wells et al, the causal bacterium of Pierce's disease of grapevine. In experi...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...
An Experimental Test of the Host-Plant Range of Nonrecombinant Strains of North American Xylella fastidiosa subsp. multiplex
Nonrecombinant strains of Xylella fastidiosa subsp. multiplex (those lacking evidence of significant intersubspecific homologous recombination) infect the xylem of a wide range of native and nonnat...
Bacterial Leaf Scorch, a New Blueberry Disease Caused by Xylella fastidiosa
Since 2004, growers and scientists have observed a disorder described as "yellow twig" or "yellow stem" affecting a major selection of southern highbush blueberry, FL 86-19, in the south Georgia bl...
Bioassay assessment of Metarhizium anisopliae (Metchnikoff) Sorokin (Deuteromycota : Hyphomycetes) against Oncometopia facialis (Signoret) (Hemiptera : Cicadellidae)
Citrus Variegated Chlorosis ( CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the effica...
BREEDING, GENETIC AND GENOMIC OF CITRUS FOR DISEASE RESISTANCE
Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture rega...
Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceralde...
Cell Wall-Degrading Enzymes Enlarge the Pore Size of Intervessel Pit Membranes in Healthy and Xylella fastidiosa-Infected Grapevines
The pit membrane (PM) is a primary cell wall barrier that separates adjacent xylem water conduits, limiting the spread of xylem-localized pathogens and air embolisms from one conduit to the next. T...
Detection of a Single-Copy Plasmid, pXFSL21, in Xylella fastidiosa Strain Stag's Leap with Two Toxin-Antitoxin Systems Using Next-Generation Sequencing
Plasmids are important genetic elements contributing to bacterial evolution and environmental adaptation. Xylella fastidiosa is a nutritionally fastidious Gram-negative bacterium causing economical...
Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence
Immunofluorescence detection was performed by tissue sectioning and membrane entrapment of Xylella fastidiosa from the inoculated hybrid selection F8909-08 (Vitis rupestris A. de Serres x V. arizon...
Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction
A sensitive and specific assay for detecting Xylella fastidiosa in potential insect vectors was developed. This assay involves immunomagnetic separation of the bacteria from the insect, followed by...
