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Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae
The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis...
Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment
Flavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. The natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture sys...
Biology and biometry of sharpshooter Homalodisca coagulata (Homoptera : Cicadellidae) reared on cowpea
Stage-specific survival, growth, developmental biology, and biometry of the sharpshooter Homalodisca coagulata (Say) were studied in the laboratory under controlled conditions of 27 ± 1°...
Characterization of the TolB-Pal trans-envelope complex from Xylella fastidiosa reveals a dynamic and coordinated protein expression profile during the biofilm development process
The intriguing roles of the bacterial Tol-Pal trans-envelope protein complex range from maintenance of cell envelope integrity to potential participation in the process of cell division. In this st...
Characterization of the Xylella fastidiosa PD1671 Gene Encoding Degenerate c-di-GMP GGDEF/EAL Domains, and Its Role in the Development of Pierce's Disease
Xylella fastidiosa is an important phytopathogenic bacterium that causes many serious plant diseases including Pierce's disease of grapevines. X. fastidiosa is thought to induce disease by colonizi...
DEVELOPMENT OF A FAST AMPLIFYRP ACCELER8 DIAGNOSTIC ASSAY FOR GRAPEVINE RED BLOTCH VIRUS
Polymerase chain reaction (PCR) is routinely used for the detection of grapevine red blotch virus (GRBV), a virus with a circular single-stranded DNA genome, in grapevine tissue. Since the preparat...
Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp pauca strain CoDiRO
Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its t...
Development of A Nested-MultiLocus Sequence Typing Approach for A Highly Sensitive and Specific Identification of Xylella fastidiosa Subspecies Directly from Plant Samples
Identification of sequence types (ST) of Xylella fastidiosa based on direct MultiLocus Sequence Typing (MLST) of plant DNA samples is partly efficient. In order to improve the sensitivity of X. f...
Development of an FTP-LAMP assay based on TaqMan real-time PCR and LAMP for the specific detection of Xylella fastidiosa De Donno and mulberry strains in both plants and insect vectors
We developed two real-time detection assays, TaqMan real-time PCR and LAMP, using primers and probe designed based on a sequence annotated to code for a Haemagglutinin-related protein (Hg) of Xyl...
DEVELOPMENT OF A POLYMERASE CHAIN-REACTION PROTOCOL FOR DETECTION OF XYLELLA-FASTIDIOSA IN PLANT-TISSUE
A 7.4-kb EcoRI fragment of genomic DNA of Xylella fastidiosa strain PCE-RR (ATCC 35879) was used as a probe and was conserved in 18 strains of Xylella. The nucleotide sequence of a 1.0-kb internal ...
Development of a real-time PCR-based method for detection of Xylophilus ampelinus
A real-time PCR MGB-probe-based detection method specific to Xylophilus ampelinus, the cause of grapevine bacterial blight, was developed. Used in combination with the DNeasy plant mini kit, the se...
Development of LAMP and Real-Time PCR Methods for the Rapid Detection of Xylella fastidiosa for Quarantine and Field Applications
Xylella fastidiosa is a regulated plant pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, a loop-mediated isothermal amplification (LAMP) and rea...
