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Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis
Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xy...
Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceralde...
Comparative Behavioral and Protein Study of Salivary Secretions in Homalodisca spp. Sharpshooters (Hemiptera: Cicadellidae: Cicadellinae)
A novel brush-induced method to physically stimulate salivation was applied to the glassy-winged and smoke tree sharpshooters (Hemiptera: Cicadellidae: Cicadellinae). This technique enabled the dir...
DETECTION AND IDENTIFICATION OF PHYTOPATHOGENIC XANTHOMONAS STRAINS BY AMPLIFICATION OF DNA-SEQUENCES RELATED TO THE HRP GENES OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA
Three pairs of oligonucleotide primers specific for different regions of the hrp gene (hypersensitive reaction and pathogenicity) cluster of Xanthomonas campestris pv. vesicatoria were designed and...
Effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (Solanum tuberosum)
Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization. The major factors contri...
Evidences of Siderophores Synthesis by Grapevine Xylella fastidiosa, Causal Agent of Pierce's Disease, through Instrumental Approaches
Siderophore molecules from grapevine Xylella fastidiosa were investigated. Such metabolites sequester iron, an essential element, from the host, making them a potential factor of pathogenicty. In a...
Intraspecific diversity of Brevibacterium linens, Corynebacterium glutamicum and Rhodococcus erythropolis based on partial 16S rDNA sequence analysis and Fourier-transform infrared (FT-IR) spectroscopy
The intraspecific diversity of 31 strains of Brevibacterium linens, 27 strains of Corynebacterium glutamicum and 29 strains of Rhodococcus erythropolis was determined by partial 16S rDNA sequence a...
Multilocus sequence type system for the plant pathogen Xylella fastidiosa and relative contributions of recombination and point mutation to clonal diversity
Multilocus sequence typing (MLST) identifies and groups bacterial strains based on DNA sequence data from (typically) seven housekeeping genes. MLST has also been employed to estimate the relative ...
Porphyromonas gingivalis DPP-7 represents a novel type of dipeptidylpeptidase
A novel dipeptidylpeptidase (DPP-7) was purified from the membrane fraction of Porphyromonas gingivalis. This enzyme, with an apparent molecular mass of 76 kDa, has the specificity for both aliphat...
The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere
Plasmid pIPO2 is a cryptic, conjugative, broad-host-range plasmid isolated from the wheat rhizosphere. it efficiently self-transfers between alpha, beta and gamma Proteobacteria and has a mobilizin...
