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A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa
A simplified protocol of subtractive hybridization based on the technique of L. M, Kunkel, A. P, Monaco, W, Middlesworth, H, D, Ochs & S, A. Latt (1985, Proc Natl Acad Sci USA, 82, 4778-4782) was u...
A Study in Entire Chromosomes of Violations of the Intra-strand Parity of Complementary Nucleotides (Chargaff's Second Parity Rule)
Chargaff's rule of intra-strand parity (ISP) between complementary mono/oligonucleotides in chromosomes is well established in the scientific literature. Although a large numbers of papers have bee...
Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis
Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing of Lep...
Distinguishing bacterial pathogens of potato using a genome-wide microarray approach
A set of 9676 probes was designed for the most harmful bacterial pathogens of potato and tested in a microarray format. Gene-specific probes could be designed for all genes of Pectobacterium atrose...
From Nucleotides to Satellite Imagery: Approaches to Identify and Manage the Invasive Pathogen Xylella fastidiosa and Its Insect Vectors in Europe
Biological invasions represent some of the most severe threats to local communities and ecosystems. Among invasive species, the vector-borne pathogen Xylella fastidiosa is responsible for a wide ...
Genome analysis of two novel Pseudomonas strains exhibiting differential hypersensitivity reactions on tobacco seedlings reveals differences in nonflagellar T3SS organization and predicted effector proteins
Genomic and Biological Analysis of Phage Xfas53 and Related Prophages of Xylella fastidiosa
We report the plaque propagation and genomic analysis of Xfas53, a temperate phage of Xylella fastidiosa. Xfas53 was isolated from supernatants of X. fastidiosa strain 53 and forms plaques on the s...
Global features of sequences of bacterial chromosomes, plasmids and phages revealed by analysis of oligonucleotide usage patterns
Oligonucleotide frequencies were shown to be conserved signatures for bacterial genomes, however, the underlying constraints have yet not been resolved in detail. In this paper we analyzed oligonuc...
Initial biochemical and functional characterization of a 5 '-nucleotidase from Xylella fastidiosa related to the human cytosolic 5 '-nucleotidase I
The 5'-nucleotidases constitute a ubiquitous family of enzymes that catalyze either the hydrolysis or the transfer of esterified phosphate at the 5' position of nucleoside monophosphates. These enz...
oriC Region and replication termination site, dif, of the Xanthomonas campestris pv. campestris 17 chromosome
A 13-kb DNA fragment containing oriC and the flanking genes thdF, orf900, yidC, rnpA, rpmH, oriC, dnaA, dnaN, recF, and gyrB was cloned from the gram-negative plant pathogen Xanthomonas campestris ...
Pathogen special: Vibrio cholerae, Pseudomonas aeruginosa and Xylella fastidiosa
One could almost say that it is the latest fashion to sequence a bacterial genome. However, this would belittle the efforts of those working on these important organisms, whose data will greatly he...
Plasmid pBP136 from Bordetella pertussis represents an ancestral form of IncP-1 beta plasmids without accessory mobile elements
The complete 41 268 bp nucleotide sequence of the IncP-1 beta plasmid pBP136 from the human pathogen Bordetella pertussis, the primary aetiological agent of whooping cough, was determined and analy...
