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16S-23S RDNA: Polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S- 23S rDN...
A geostatistical fusion approach using UAV data for probabilistic estimation of Xylella fastidiosa subsp. pauca infection in olive trees.
Xylella fastidiosa is one of the most destructive plant pathogenic bacteria worldwide, affecting more than 500 plant species. In Apulia region (southeastern Italy), X. fastidiosa subsp. pauca (Xf...
A multigene phylogenetic study of clonal diversity and divergence in North American strains of the plant pathogen Xylella fastidiosa
Xylella fastidiosa is a pathogen that causes leaf scorch and related diseases in over 100 plant species, including Pierce's disease in grapevines (PD), phony peach disease (PP), plum leaf scald (PL...
Analysis of resistance to Xylella fastidiosa within a hybrid population of Pera sweet orange x Murcott tangor
Resistance to Xylella fastidiosa was evaluated within a population of 20 interspecific hybrids of Pera sweet orange and Murcott tangor under greenhouse conditions. Efficiency of inoculation, multip...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...
A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa
A simplified protocol of subtractive hybridization based on the technique of L. M, Kunkel, A. P, Monaco, W, Middlesworth, H, D, Ochs & S, A. Latt (1985, Proc Natl Acad Sci USA, 82, 4778-4782) was u...
Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X-cynarae
The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity in Xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. This RAPD analysis...
Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridizations
Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped in...
A zinc, copper and citric acid biocomplex shows promise for control of Xylella fastidiosa subsp pauca in olive trees in Apulia region (southern Italy)
The bacterium Xylella fastidiosa subsp. pauca is associated with the “olive quick decline syndrome” in the Apulia region of southern Italy. To investigate control of this phytopathogen, a compound ...
BACTERIAL DISEASES THAT MAY OR DO EMERGE, WITH (POSSIBLE) ECONOMIC DAMAGE FOR EUROPE AND THE MEDITERRANEAN BASIN: NOTES ON EPIDEMIOLOGY, RISKS, PREVENTION AND MANAGEMENT ON FIRST OCCURRENCE
Bacterial diseases are difficult to control (both chemically and biologically), and are restrained primarily by preventive measures. Most important risk factors for the introduction or spread of ba...
Combination of Chromogenic Differential Medium and estA-Specific PCR for Isolation and Detection of Phytopathogenic Xanthomonas spp.
A xanthomonad differential medium (designated Xan-D medium) was developed, on which streaks and colonies of xanthomonads, including 13 species of the genus Xanthomonas, turned wet-shining yellow-gr...
Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
Bacterial leaf scorch, caused by Xylella fastidiosa, is a major threat to blueberry production in the southeastern United States. Management of this devastating disease is challenging and often req...
