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An engineered innate immune defense protects grapevines from Pierce disease
We postulated that a synergistic combination of two innate immune functions, pathogen surface recognition and lysis, in a protein chimera would lead to a robust class of engineered antimicrobial th...
A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone pepton...
Bacteriocins from plant pathogenic bacteria
Many bacteria produce antimicrobial substances such as nonribosomally synthesized antibiotics and ribosomally synthesized proteinaceous compounds referred to as bacteriocins. Secretion of antimicro...
Characterization of Novel Virulent Broad-Host-Range Phages of Xylella fastidiosa and Xanthomonas
The xylem-limited bacterium Xylella fastidiosa is the causal agent of several plant diseases, most notably Pierce's disease of grape and citrus variegated chlorosis. We report the isolation and cha...
Differential Susceptibility of Xylella fastidiosa Strains to Synthetic Bactericidal Peptides
The kinetics of cell inactivation and the susceptibility of Xylella fastidiosa subspecies fastidiosa, multiplex, and pauca to synthetic antimicrobial peptides from two libraries (CECMEL11 and CYC...
Effect of five antimicrobial peptides on the growth of Agrobacterium tumefaciens, Escherichia coli and Xylella fastidiosa
Five lytic peptides, cecropin A (CA), cecropin B (CB), magainin I (MI), magainin II (MII) and Shiva-1 (a synthetic analog of CB), were evaluated for their antimicrobial activity against Escherichia...
Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa
In the xylem vessels of susceptible hosts, such as citrus trees, Xylella fastidiosa forms biofilm-like colonies that can block water transport, which appears to correlate to disease symptoms. Besid...
Influence of Vitis xylem fluid and xylem fluid plus cecropin on growth of Xylella fastidiosa
Colony growth of Xylella fastidiosa (UCLA ID and STL PD strains) was quantified after incubation for 48 h in xylem fluid of Vitis rotundifolia Michx. cv. Noble and Vitis vinifera L. cv. Chardonnay....
In vitro activities of antibiotics and antimicrobial peptides against the plant pathogenic bacterium Xylella fastidiosa
The objective of this study was to evaluate the effectiveness of antibiotics and antimicrobial peptides against 10 strains of Xylella fastidiosa. The minimal inhibitory concentration (MIC) of 12 a...
Screening and identification of BP100 peptide conjugates active againstXylella fastidiosausing a viability-qPCR method
Xylella fastidiosa is one of the most harmful bacterial plant pathogens worldwide, causing a variety of diseases, with huge economic impact to agriculture and environment. Although it has been ex...
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts
Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa...
