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A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers

Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...

Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction

A sensitive and specific assay for detecting Xylella fastidiosa in potential insect vectors was developed. This assay involves immunomagnetic separation of the bacteria from the insect, followed by...

Real-time PCR and its application for rapid plant disease diagnostics

Rapid-cycle real-time polymerase chain reaction (PCR) methods may revolutionize the manner in which plant pathogens are identified and diseases are diagnosed. As the genomics age progresses and mor...