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Acquisition and Evolution of Plant Pathogenesis-Associated Gene Clusters and Candidate Determinants of Tissue-Specificity in Xanthomonas
Background: Xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. Collectively, members cause diseases on over 392 plant species. Individually, they exhibit marked host- a...
An Experimental Test of the Host-Plant Range of Nonrecombinant Strains of North American Xylella fastidiosa subsp. multiplex
Nonrecombinant strains of Xylella fastidiosa subsp. multiplex (those lacking evidence of significant intersubspecific homologous recombination) infect the xylem of a wide range of native and nonnat...
Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobactetium avium N85 and preliminary characterization of DhmA
Haloalkane dehalogenases are microbial enzymes that catalyze cleavage of the carbon-halogen bond by a hydrolytic mechanism. Until recently, these enzymes have been isolated only from bacteria livin...
GENETIC DISTANCE MAY UNDERLIE VIRULENCE DIFFERENCES AMONG ISOLATES OF A BACTERIAL PLANT PATHOGEN
Certain pathogens evolve with their hosts in a stepwise arms race of virulence and resistance, mediated by one or a few genetic loci in their genomes. Other pathogens may retain the ability to colo...
Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean
Host specialization is a hallmark of numerous plant pathogens including bacteria, fungi, oomycetes and viruses. Yet, the molecular and evolutionary bases of host specificity are poorly understood. ...
Ohr plays a central role in bacterial responses against fatty acid hydroperoxides and peroxynitrite
Organic hydroperoxide resistance (Ohr) enzymes are unique Cysbased, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroper-oxides...
PCR Species-Specific Primers for Molecular Gut Content Analysis to Determine the Contribution of Generalist Predators to the Biological Control of the Vector of Xylella fastidiosa
Quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in Pantoea stewartii subspecies stewartii
The phytopathogenic bacterium Pantoea stewartii subsp. stewartii synthesizes stewartan exo/capsular polysaccharide (EPS) in a cell density-dependent manner governed by the Esal/EsaR quorum-sensing ...
The role of lipopolysaccharide in virulence and host specificity of Xylella fastidiosa
This project focuses on the lipopolysaccharide (LPS) component of the outer membrane of Xylella fastidiosa (Xf). In particular, we are investigating if the O-antigen portion of this molecule contri...
Xylella fastidiosa evolution, determinants of host plant specificity, and pathogen adaptation to novel hosts
Despite the global importance of the bacterium Xylella fastidiosa, there is limited understanding of what determines its host specificity, and how this pathogen adapts to new host plant species. An...