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Acquisition and Evolution of Plant Pathogenesis-Associated Gene Clusters and Candidate Determinants of Tissue-Specificity in Xanthomonas
Background: Xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. Collectively, members cause diseases on over 392 plant species. Individually, they exhibit marked host- a...
Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment
Flavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. The natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture sys...
Breaking dogmas: the plant vascular pathogen Xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome
Xanthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the Hrp type III secretion system that is used by many Gram-negative bacteria to colonize their host. Until now, this p...
Confirmation of Xylella fastidiosa infecting grapes Vitis vinifera in Costa Rica
In 2003, symptoms of Pierce Disease (PD) were observed in two vineyards established in two different localities in San Jose province (Santa Ana and La Uruca) and in La Garita, Alajuela province. Tw...
Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence
Immunofluorescence detection was performed by tissue sectioning and membrane entrapment of Xylella fastidiosa from the inoculated hybrid selection F8909-08 (Vitis rupestris A. de Serres x V. arizon...
Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp pauca strain CoDiRO
Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its t...
DEVELOPMENT OF A POLYMERASE CHAIN-REACTION PROTOCOL FOR DETECTION OF XYLELLA-FASTIDIOSA IN PLANT-TISSUE
A 7.4-kb EcoRI fragment of genomic DNA of Xylella fastidiosa strain PCE-RR (ATCC 35879) was used as a probe and was conserved in 18 strains of Xylella. The nucleotide sequence of a 1.0-kb internal ...
Direct tissue blot immunoassay for detection of Xylella fastidiosa in olive trees
A direct tissue blot immunoassay (DTBIA) technique has been compared with ELISA and PCR for detection of Xylella fastidiosa in olive trees from Apulia (southern Italy). Fresh cross-sections of youn...
ISOLATION AND PRELIMINARY CHARACTERIZATION OF EXTRACELLULAR PROTEASES PRODUCED BY STRAINS OF XYLELLA-FASTIDIOSA FROM GRAPEVINES
Virulent and weakly virulent grape strains of Xylella fastidiosa grew well on PD3 amended with gelatin and produced zones of hydrolysis on this medium, indicating the presence of proteolytic activi...
Methylobacterium-Induced Endophyte Community Changes Correspond with Protection of Plants against Pathogen Attack
Plant inoculation with endophytic bacteria that normally live inside the plant without harming the host is a highly promising approach for biological disease control. The mechanism of resistance in...
Novel Tetraplex Quantitative PCR Assays for Simultaneous Detection and Identification of Xylella fastidiosa Subspecies in Plant Tissues
Xylella fastidiosa (Xf) is an insect-borne bacterium confined to the xylem vessels of plants. This plant pathogen has a broad host range estimated to 560 plant species. Five subspecies of the patho...
On-site detection of Xylella fastidiosa in host plants and in "spy insects" using the real-time loop-mediated isothermal amplification method
A recent severe outbreak of Xylella fastidiosa associated with 'olive quick decline syndrome' (OQDS) was reported in Apulia (Southern Italy). In this study an on-site real-time loop-mediated isothe...
