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A Short Protocol for Gene Knockout and Complementation in Xylella fastidiosa Shows that One of the Type IV Pilin Paralogs (PD1926) Is Needed for Twitching while Another (PD1924) Affects Pilus Number and Location
Twitching motility is one of the major virulence factors of the plantpathogenic bacterium Xylella fastidiosa, and it is mediated by type IV pili (TFP) that are present at one of the cell poles. Gen...
BREEDING, GENETIC AND GENOMIC OF CITRUS FOR DISEASE RESISTANCE
Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture rega...
Citrus plastid-related gene profiling based on expressed sequence tag analyses
Plastid-related sequences, derived from putative nuclear or plastome genes, were searched in a large collection of expressed sequence tags (ESTs) and genomic sequences from the Citrus Biotechnology...
Effects of DNA Size on Transformation and Recombination Efficiencies in Xylella fastidiosa
Horizontally transferred DNA acquired through transformation and recombination has the potential to contribute to the diversity and evolution of naturally competent bacteria. However, many differen...
EXTRACELLULAR GENOMIC DNA MEDIATES ENHANCEMENT OF XYLELLA FASTIDIOSA BIOFILM FORMATION IN VITRO
Xylella fastidiosa (Xf) produces extracellular DNA in PD3 liquid medium. This extracellular DNA could enhance biofilm formation, a factor in successful establishment of Xf in planta. The relative a...
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformatio...
Natural Competence and Recombination in the Plant Pathogen Xylella fastidiosa
Homologous recombination is one of many forces contributing to the diversity, adaptation, and emergence of pathogens. For naturally competent bacteria, transformation is one possible route for the ...
Natural Competence of Xylella fastidiosa Occurs at a High Frequency Inside Microfluidic Chambers Mimicking the Bacterium's Natural Habitats
Xylella fastidiosa is a xylem-limited bacterium that is the causal agent of emerging diseases in a number of economically important crops. Genetic diversity studies have demonstrated homologous rec...
Novel candidate virulence factors in rice pathogen Xanthomonas oryzae pv. oryzicola as revealed by mutational amalysis
Bacterial leaf streak, caused by Xanthomonas oryzae pv. oryzicola, is an important disease of rice. Transposon-mediated mutational analysis of the pathogen with a quantitative assay revealed candid...
Site-directed gene disruption in Xylella fastidiosa
As a first approach to generate mutations by DNA insertion, we have developed a shuttle vector, called pSP3. which replicates both in Escherichia coli and in Xylella. Vector pSP3 was constructed by...
Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1
The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report re...
Stable transformation of the Xylella fastidiosa citrus variegated chlorosis strain with oriC plasmids
Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e,g,, grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fasti...
