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Conjugative Plasmid Transfer in Xylella fastidiosa Is Dependent on tra and trb Operon Functions
The insect-transmitted plant pathogen Xylella fastidiosa is capable of efficient horizontal gene transfer (HGT) and recombination. Natural transformation occurs at high rates in X. fastidiosa, but ...
High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X-campestris pv. campestris generated by an efficient transposon mutagenesis system
A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highl...
Phylogeny of Replication Initiator Protein TrfA Reveals a Highly Divergent Clade of Incompatibility Group P1 Plasmids
Incompatibility group P1 (IncP-1) plasmid diversity was evaluated based on replication initiator protein (TrfA) phylogeny. A new and highly divergent clade was identified. Replication assays indica...
Plasmid pBP136 from Bordetella pertussis represents an ancestral form of IncP-1 beta plasmids without accessory mobile elements
The complete 41 268 bp nucleotide sequence of the IncP-1 beta plasmid pBP136 from the human pathogen Bordetella pertussis, the primary aetiological agent of whooping cough, was determined and analy...
The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa
Employing the biparental exogenous plasmid isolation method, conjugative plasmids conferring mercury resistance were isolated from the microbial community of the rhizosphere of field grown alfalfa ...
Transposon Tn1721 distribution among strains of Xylella fastidiosa
Transposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the ge...
