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ACQUISITION AND RETENTION OF XYLELLA-FASTIDIOSA BY AN EFFICIENT VECTOR, GRAPHOCEPHALA-ATROPUNCTATA
The blue-green sharpshooter (BGSS), Graphocephala atropunctata (Signoret), is an efficient vector of Xylella fastidiosa Wells et al, the causal bacterium of Pierce's disease of grapevine. In experi...
DEVELOPMENT OF A POLYMERASE CHAIN-REACTION PROTOCOL FOR DETECTION OF XYLELLA-FASTIDIOSA IN PLANT-TISSUE
A 7.4-kb EcoRI fragment of genomic DNA of Xylella fastidiosa strain PCE-RR (ATCC 35879) was used as a probe and was conserved in 18 strains of Xylella. The nucleotide sequence of a 1.0-kb internal ...
DISTRIBUTION OF XYLELLA-FASTIDIOSA WITHIN ROOTS OF PEACH
The distribution of Xylella fastidiosa, the bacterium that causes phony peach disease, was studied in roots of 10 Flordaking peach scions grafted on Nemaguard rootstock. X. fastidiosa was detected ...
EVALUATION OF A RAPID ELISA TEST KIT FOR DETECTION OF XYLELLA-FASTIDIOSA IN LANDSCAPE TREES
Leaf scorch symptoms on landscape trees infected with Xylella fastidiosa are often confused with similar symptoms caused by stress factors. Because the isolation of X. fastidiosa from trees is diff...
GENETIC-VARIATION AMONG XYLELLA-FASTIDIOSA STRAINS
Restriction fragment length polymorphism (RFLP) analysis was used to determine similarities and differences in DNAs among 24 strains of Xylella fastidosa from eight different hosts. Two genomic lib...
ISOLATION AND GROWTH OF STRAINS OF XYLELLA-FASTIDIOSA FROM INFECTED GRAPEVINES ON NUTRIENT AGAR MEDIA
Two grape strains (FC and C) of the causal organism of Pierce’s disease (PD) of grapevine, Xylella fastidiosa, were isolated from infected grape petioles onto PD3 medium, nutrient agar (NA), and NA...
ISOLATION AND PRELIMINARY CHARACTERIZATION OF EXTRACELLULAR PROTEASES PRODUCED BY STRAINS OF XYLELLA-FASTIDIOSA FROM GRAPEVINES
Virulent and weakly virulent grape strains of Xylella fastidiosa grew well on PD3 amended with gelatin and produced zones of hydrolysis on this medium, indicating the presence of proteolytic activi...
ISOLATION, CULTIVATION, AND PATHOGENICITY OF XYLELLA-FASTIDIOSA, THE CAUSAL BACTERIUM OF PEAR LEAF SCORCH DISEASE IN TAIWAN
Leaf scorch symptoms occurred in 12-20.6% of the pear trees (Pyrus pyrifolia) in the low-altitude areas of central Taiwan in 1990. Symptoms appeared on leaves in early July and stayed until the lea...
MULTIPLICATION AND MOVEMENT OF XYLELLA-FASTIDIOSA WITHIN GRAPEVINE AND 4 OTHER PLANTS
Xylella fastidiosa, a xylem-limited bacterium that causes Pierce's disease of grapevine and other plant diseases, has numerous symptomless hosts. The multiplication and spread of X. fastidiosa with...
MULTIPLICATION AND TRANSLOCATION OF XYLELLA-FASTIDIOSA IN PETIOLES AND STEMS OF GRAPEVINE RESISTANT, TOLERANT, AND SUSCEPTIBLE TO PIERCES DISEASE
Petioles of Vitis vinifera ‘French Colombard’ (susceptible to Pierce’s disease), and V. rotundifolia ‘Carlos’ (tolerant) and ‘Noble’ (resistant) were inoculated with virulent strain FC of Xylella f...
NATURAL HOSTS OF XYLELLA-FASTIDIOSA IN FLORIDA
Fluorescence microscopy, enzyme-linked immunosorbent assay, and direct culturing were used to determine natural infection of various weeds and wild plants with Xylella fastidiosa, a xylem-limited b...
PATHOGENICITY OF XYLELLA-FASTIDIOSA IN AMERICAN ELM AND FAILURE OF RECIPROCAL TRANSMISSION BETWEEN STRAINS FROM ELM AND SYCAMORE
Pathogenicity of Xylella fastidiosa was demonstrated in American elm seedlings. Ten 4-mo-old seedlings were stem-inoculated with a pure culture of X. fastidiosa obtained from a naturally infected e...
