Detection of Xylella fastidiosa Wells et al. by enzyme-linked immunosorbent assay indicated that plums (Prunus hybrids) had higher absorbance values than peaches [Prunus persica (L.) Batsch]. The slip-budded trees had lower readings than those that were chip budded; however, the scion x method interaction was significant. Further comparison of slip vs. chip budding indicated that the lower absorbance value of slip budding occurred in plums only; there was no difference between budding methods in peach.