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A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
Detection of Xylella fastidiosa in citrus plants and insect vectors. Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp wa...
Comparative genomic characterization of citrus-associated Xylella fastidiosa strains
The xylem-inhabiting bacterium Xylella fastidiosa (Xf) is the causal agent of Pierce's disease (PD) in vineyards and citrus variegated chlorosis (CVC) in orange trees. Both of these economically-de...
Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants
Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resist...
Effects of citrus and avocado irrigation and nitrogen-form soil amendment on host selection by adult Homalodisca vitripennis (Hemiptera : Cicadellidae)
Host plant water status is thought to influence dispersal of the xylophagous leafhopper Homalodisca vitripennis Germar, especially where plants are grown under high evaporative demand. Preference b...
Evaluation of the genetic structure of Xylella fastidiosa populations from different Citrus sinensis varieties
Xylella fastidiosa was isolated from sweet orange plants (Citrus sinensis) grown in two orchards in the northwest region of the Brazilian state of Sao Paulo. One orchard was part of a germ plasm fi...
Feeding and survival of citrus sharpshooters (Hemiptera : Cicadellidae) on host plants
The liquid excretion and survival of the sharpshooters Dilobopterus costalimai Young and Oncometopia facialis (Signoret), vectors of Xylella fastidiosa in citrus, were measured on various host plan...
Genetic structure and biology of Xylella fastidiosa strains causing disease in citrus and coffee in Brazil
Xylella fastidiosa is a vector-borne, plant-pathogenic bacterium that causes disease in citrus (citrus variegated chlorosis [CVC]) and coffee (coffee leaf scorch [CLS]) plants in Brazil. CVC and CL...
Populations of sharpshooters in two citrus groves in east-central Florida as indicated by yellow sticky card traps
Three sharpshooter species were captured on yellow sticky card traps in two citrus groves in east-central Florida: Homalodisca insolita, H. vitripennis and Oncometopia nigricans. Homalodisca vitrip...
Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C-limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry
A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a r...
The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants
The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC) and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (...
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival ...
