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BACTERIAL DISEASES THAT MAY OR DO EMERGE, WITH (POSSIBLE) ECONOMIC DAMAGE FOR EUROPE AND THE MEDITERRANEAN BASIN: NOTES ON EPIDEMIOLOGY, RISKS, PREVENTION AND MANAGEMENT ON FIRST OCCURRENCE
Bacterial diseases are difficult to control (both chemically and biologically), and are restrained primarily by preventive measures. Most important risk factors for the introduction or spread of ba...
Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
Bacterial leaf scorch, caused by Xylella fastidiosa, is a major threat to blueberry production in the southeastern United States. Management of this devastating disease is challenging and often req...
Comparison of conventional and novel molecular diagnostic methods for detection of Xylella fastidiosa from insect vectors
The efficiency of three diagnostic methods, i.e. PCR, real-time PCR and LAMP, for detection of Xylella fastidiosa (Xf) genomic DNA from Philaenus spumarius (Ps) and Neophilaenus campestris (Nc) i...
Development of an FTP-LAMP assay based on TaqMan real-time PCR and LAMP for the specific detection of Xylella fastidiosa De Donno and mulberry strains in both plants and insect vectors
We developed two real-time detection assays, TaqMan real-time PCR and LAMP, using primers and probe designed based on a sequence annotated to code for a Haemagglutinin-related protein (Hg) of Xyl...
Development of a real-time PCR-based method for detection of Xylophilus ampelinus
A real-time PCR MGB-probe-based detection method specific to Xylophilus ampelinus, the cause of grapevine bacterial blight, was developed. Used in combination with the DNeasy plant mini kit, the se...
Development of LAMP and Real-Time PCR Methods for the Rapid Detection of Xylella fastidiosa for Quarantine and Field Applications
Xylella fastidiosa is a regulated plant pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, a loop-mediated isothermal amplification (LAMP) and rea...
Diagnostic training Workshop: detection of Xylella fastidiosa
Protocols for the detection of Xylella fastidiosa, as prepared for the DIagnostic training Workshop organised by the Science and Advice for Scottish Agriculture (SASA) in September 2016
Enhanced Reliability and Accuracy for Field Deployable Bioforensic Detection and Discrimination of Xylella fastidiosa subsp pauca, Causal Agent of Citrus Variegated Chlorosis Using Razor Ex Technology and TaqMan Quantitative PCR
A reliable, accurate and rapid multigene-based assay combining real time quantitative PCR (qPCR) and a Razor Ex BioDetection System (Razor Ex) was validated for detection of Xylella fastidiosa subs...
EPPO DIagnostic Protocol PM 7/141 (1) Philaenus spumarius, Philaenus italosignus and Neophilaenus campestris
The Standard describes a diagnostic protocol for the vectors of Xylella fastidiosa Philaenus spumarius, Philaenus italosignus and Neophilaenus campestris.
Approved in 2019-06.
EPPO Diagnostic Protocol PM 7/24 (2) Xylella fastidiosa
The Standard describes a diagnostic protocol for Xylella fastidiosa.
First approved in 2004-09. Revised in 2016-09.
EPPO Diagnostic Protocol PM 7/24 (3) Xylella fastidiosa
The Standard describes a diagnostic protocol for Xylella fastidiosa.
First approved in 2004-09. Revised in 2016-09 and 2018-04.
EPPO Diagnostic Protocol PM 7/24 (4) Xylella fastidiosa
The Standard describes a diagnostic protocol for Xylella fastidiosa.
First approved in 2004-09. Revised in 2016-09, 2018-04 and 2019–05.