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Analysis of the genome-wide variations among multiple strains of the plant pathogenic bacterium Xylella fastidiosa

The Gram-negative, xylem-limited phytopathogenic bacterium Xylella fastidiosa is responsible for causing economically important diseases in grapevine, citrus and many other plant species. Despite i...

Anatomy and histology of reproductive organs of female Homalodisca coagulata (Hemiptera : Cicadellidae : Proconiini), with special emphasis on categorization of vitellogenic oocytes

The anatomy and histology of female Homalodisca coagulata (Say) (Hemiptera: Ci-cadellida: Proconiini) reproductive organs is described using light microscopy. The reproductive organs of H. coagulat...

Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover

HD-GYP is a protein domain of unknown biochemical function implicated in bacterial signaling and regulation. In the plant pathogen Xanthomonas campestris pv. campestris, the synthesis of virulence ...

Detection and characterization of protease secreted by the plant pathogen Xylella fastidiosa

Xylella fastidiosa is a pathogenic bacterium found in several plants. These bacteria secrete extracellular proteases into the culture broth as visualized in sodium-dodecytsulfate potyacrytamide act...

Disruption of Xylella fastidiosa CVC gumB and gumF genes affects biofilm formation without a detectable influence on exopolysaccharide production

Xylella fastidiosa causes citrus variegated chlorosis (CVC), a destructive disease of citrus. Xylella fastidiosa forms a biofilm inside plants and insect vectors. Biofilms are complex structures in...

Fast, easy and efficient DNA extraction and one-step polymerase chain reaction for the detection of Xylella fastidiosa in potential insect vectors

A quick, simple and efficient procedure for detecting Xylella fastidiosa in potential insect vectors is described. The procedure employs a commercially available DNeasy tissue kit for the extractio...

First report of a Proconiine sharpshooter, Anacuerna centrolinea (Hemiptera : Cicadellidae), in chile, with notes on its biology, host plants, and egg parasitoids

The first representative of the leafhopper tribe Proconiini (subfamily Cicadellinae), Anacuerna centrolinea (Melichar) is reported herein from the Tarapaca region in northern Chile. This species wa...

Genetic differences between blight-causing Erwinia species with differing host specificities, identified by suppression subtractive hybridization

PCR-based subtractive hybridization was used to isolate sequences from Erwinia amylovora strain Ea110, which is pathogenic on apples and pears, that were not present in three closely related strain...

Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa

Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fasti...

GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange

The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformatio...

Identification of mechanisms mediating cold therapy of Xylella fastidiosa-infected grapevines

We are attempting to characterize the biochemical factors that mediate the cold curing phenomenon of grapevines infected with Xylella fastidiosa (Xf). We are working towards completing project obje...

In vitro activities of antibiotics and antimicrobial peptides against the plant pathogenic bacterium Xylella fastidiosa

The objective of this study was to evaluate the effectiveness of antibiotics and antimicrobial peptides against 10 strains of Xylella fastidiosa. The minimal inhibitory concentration (MIC) of 12 a...